Ders Detayları
| Code | Name | Type | Year | Semester | ECTS |
| 623002042015 | Molecular Biological Methods | Zorunlu | 2 | 4 | 5.00 |
Objectives of the Course
This course aims to provide the students the basic principles and methods used in molecular biology laboratories.
Name of Lecturer(s)
Dr. Öğr. Üyesi Sevgin DEĞİRMENCİOĞLU
Learning Outcomes
| 1 | Describe the field of molecular biology |
| 2 | Comprehend the laboratory safety issues |
| 3 | Recognise and know the laboratory equipment |
| 4 | Analyze the methods of isolation and purification methods in molecular biology |
| 5 | Explain the methods of analyzing DNA in molecular biology |
Mode of Delivery
Dr. Öğr. Üyesi Sevgin DEĞİRMENCİOĞLU
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Molecular Biological Methods includes the basic principles and methods of molecular biology. In this context laboratory safety rules are handled, the equipments in the lab are introduced, solution preparation is reviewed and the basic methods used in the molecular biology lab such as separation, purification and analyses of DNA, RNA and proteins are explained. Additionally biotechnological issues are discussed.
Weekly Detailed Course Contents
| 1 | Introduction to Molecular Biology |
| 2 | Safety in a molecular biology laboratory |
| 3 | Equipments in a molecular biology laboratory |
| 4 | The cell |
| 5 | Genetic material (Chromosome) |
| 6 | Genetic material (DNA) |
| 7 | Midterm Exam Week |
| 8 | Midterm Exam Week |
| 9 | Cell Lysis and Homogenization |
| 10 | Isolation and Purification Methods |
| 11 | Spectral Analysis |
| 12 | Electrophoresis |
| 13 | DNA Amplification (PCR) |
| 14 | Analyses of DNA and Biotechnology |
| 15 | Final Exam Week |
| 16 | Final Exam Week |
Recommended or Required Reading
1. Moleküler Biyolojide Kullanılan Yöntemler, Güler Temizkan, Nazlı Arda, 2004, Nobel Yayın Dağıtım.2. Biyoloji Laboratuvarının Temelleri, Elçin, Erkoç, Atik, Selvi, Sarıkaya, Öztekin, 2010, Palme Yayınevi.3. Molekülden Hücreye, Dokudan Fizyolojiye Biyoloji Deneyleri, Elçin, Erkoç, Atik, Selvi, Sarıkaya, Öztekin, 2010, Palme Yayınevi.
Assessment Methods and Criteria
| Yarıyıl (Yıl) İçi Etkinlikleri | 40 |
| Yarıyıl (Yıl) Sonu Etkinlikleri | 60 |
| 100 |
Language of Instruction
Turkish
Work Placement(s)
None
Workload Calculation
| Activities | Count | WORKLOAD (hours) | TOTAL WORKLOAD (hours) |
|---|---|---|---|
| Ara Sınav | 1 | 1 | 1 |
| Final Sınav | 1 | 1 | 1 |
| Derse Katılım | 12 | 1 | 12 |
| 12 | 2 | 24 | |
| 1 | 2 | 2 | |
| Soru-Yanıt | 11 | 2 | 22 |
| 1 | 2 | 2 | |
| 10 | 2 | 20 | |
| 12 | 1 | 12 | |
| Bireysel Çalışma | 12 | 2 | 24 |
| 1 | 6 | 6 | |
| Ara Sınav İçin Bireysel Çalışma | 1 | 6 | 6 |
| Final Sınavı içiin Bireysel Çalışma | 1 | 10 | 10 |
| 142 |